antibodies against nrf2  (Servicebio Inc)

Journal: Materials Today Bio

Article Title: Bioinspired lipid droplets nanoplatform for periodontitis therapy: Integrated antibacterial, mitochondrial repair, and immunomodulatory functions

doi: 10.1016/j.mtbio.2026.102808

Figure Lengend Snippet: Exploration of the anti-inflammatory and antioxidant mechanisms of GA@LDs-CRAMP in BMDMs . A) Immunofluorescence images of Nrf2 (red) and nuclei (blue) in LPS-stimulated BMDMs. Scale bar: 10 μm. B) Quantitative analysis of the Pearson colocalization coefficient between Nrf2 red fluorescence and DAPI-stained nuclear blue fluorescence (n = 5). C) Western blot validation and semiquantitative analysis of macrophage polarization markers (iNOS, Arg1) and inflammatory cytokines (IL-6). D) Western blot validation and semiquantitative analysis of relative expression of NF-κB pathway components (p65, p-p65, IκBα, p-IκBα) (n = 3). E) Western blot validation and semiquantitative analysis of relative expression of Nrf2 pathway components (Nrf2, Keap1, HO-1, NQO1) (n = 3). ns > 0.05, ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, and ∗∗∗∗ p < 0.0001. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: Primary antibody against Nrf2 (1:50, Abmart, #T55136) was added and incubated overnight at 4 °C, followed by rewarming at room temperature for 15 min and three washes with PBS.

Techniques: Immunofluorescence, Fluorescence, Staining, Western Blot, Biomarker Discovery, Expressing

Journal: iScience

Article Title: USP1 promotes hepatocellular carcinoma progression by modulating mitophagy via stabilizing MCM3 to regulate the Keap1-Nrf2 axis

doi: 10.1016/j.isci.2026.114927

Figure Lengend Snippet: MCM3 regulates mitophagy by influencing the expression and localization of Nrf-2 (A) WB used to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells. (B) WB was used to assess the impact of MCM3 knockdown on Nucleus Nrf2 in Huh7 and LM3 cells. (C) IF was used to evaluate the impact of MCM3 knockdown on Nrf2 in Huh7 and LM3 cells ( n = 3 biologically independent experiments). (D) WB was used to measure the impact of MCM3 knockdown on HMOX-1 and NQO1 expression in Huh7 and LM3 cells, with or without THBQ. (E) WB was performed to detect the impact of MCM3 knockdown on P62, LC3B II/I, TOMM20, FUNDC1, Parkin, and PINK1 in Huh7 and LM3 cells, with or without THBQ. Data are presented as mean ± SD. ∗∗∗∗ p < 0.0001; ∗∗∗ p < 0.001; ∗∗ p < 0.01; ∗ p < 0.05, ns p ≥ 0.05. For (A), (D), and (E), statistical analysis was performed using a one-way ANOVA ( n = 3 biologically independent experiments); For B, statistical analysis was performed using a two-tailed unpaired Student’s t test ( n = 3 biologically independent experiments).

Article Snippet: Following three washes with PBS, the cells were blocked with an immunofluorescence blocking solution (Beyotime) at 37°C for 1 h. Subsequently, the cells were incubated with a primary antibody against Nrf-2 (Proteintech, #16396-1-AP, 1:50 dilution) at 4°C overnight.

Techniques: Expressing, Knockdown, Two Tailed Test